Transcriptome-Wide Integrated Analysis of the PgGT25-04 Gene in Controlling Ginsenoside Biosynthesis in Panax ginseng

Panax ginseng is a valuable medicinal herb of the Araliaceae family with various pharmacological activities. The Trihelix transcription factors involved in growth and secondary metabolic processes plants, but no studies have been reported on involvement genes ginseng. In this study, weighted co-expression network analysis, correlation analysis between PgGTs ginsenosides key enzyme genes, interaction were used to screen out PgGT25-04 gene, which was negatively correlated ginsenoside synthesis. Using ABA treatment hair roots, PgGT found respond signals. Analysis sequence characteristics expression pattern gene revealed that its spatiotemporally specific. interfering vector pBI121-PgGT25-04 containing constructed, adventitious roots transformed using Agrobacterium-mediated method obtain positive hairy root monocot line. saponin contents measured by HPLC, changes detected via fluorescence quantitative RT-PCR. These results preliminarily identified role metabolism Jilin provide theoretical basis for study